Insertion junction: CLIP2.064178_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.064178
Chromosome:chromosome 12
Location:191262
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre12.g484750 CLH2 Chlorophyllase I; (1 of 3) 3.1.1.14 - Chlorophyllase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAGATGGCGGTTGAAGGTCGGACCGCAGGCGTCCAGACCGCAGGCATG
Internal bar code:ACCAGTAGCTAGCAGGGACCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:238
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCACGTCATGTCATGTCA
Suggested primer 2:GGAGGGGGAAGGGGAGTATT