Insertion junction: CLIP2.064183_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.064183
Chromosome:chromosome 3
Location:554174
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145247 TEF11 S-isoprenylcysteine O-methyltransferase; (1 of 3) 2.1.1.100 - Protein-S-isoprenylcysteine O-methyltransferase / Prenylcysteine carboxyl methyltransferase outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTATGCGTGTAAAATAAGAGTATGGGAGCGCTGGGATTGTATGCGGAGA
Internal bar code:ACCATGGTCTCTCTTGGGTGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1320
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CAAAGACGCTTTCTGCCCAC
Suggested primer 2:TCCTTGGGATAGAGACGGCA