| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064187 |
| Chromosome: | chromosome 2 |
| Location: | 4961424 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110400 | (1 of 1) PTHR12732:SF0 - PCI DOMAIN-CONTAINING PROTEIN 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTCTATGGCCAAGCTCTTCTAGCTGCAGGCCACGTGTTCCACCGCAAC |
| Internal bar code: | GCTCTTCTCAACTTTGGACATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5030 |
| LEAP-Seq percent confirming: | 97.3451 |
| LEAP-Seq n confirming: | 110 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 113 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGTAAACCGCCTTCTCA |
| Suggested primer 2: | CCCTACGTACCGCTAAGCAG |