Insertion junction: CLIP2.064202_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.064202
Chromosome:chromosome 11
Location:919039
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467656 (1 of 9) IPR007112//IPR009009 - Expansin/pollen allergen, DPBB domain // RlpA-like double-psi beta-barrel domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGCTGCTTCGGCTGTCGTGACGTGATGGTTCTACCTGCTCCTCGACA
Internal bar code:TTCTTTCTGCTCACCGCTGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1573
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:TCTCCACCCCCAAAATACGC
Suggested primer 2:ACTGACAGCATCGACAGCAA