Insertion junction: CLIP2.064208_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.064208
Chromosome:chromosome 7
Location:1140703
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g320800 (1 of 1) PF09468 - Ydr279p protein family (RNase H2 complex component) (RNase_H2-Ydr279) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCTCAATTCCATAACAAGCGCTCTGCACACTAAGCCCCCATTCCGTAC
Internal bar code:GACACAGGCGGGCGCCGAAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2087
LEAP-Seq percent confirming:91.4286
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGGACGATTGGATTGCGA
Suggested primer 2:AAGCGTTGCCTTTTGCGATT