Insertion junction: CLIP2.064217_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.064217
Chromosome:chromosome 7
Location:5661110
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre07.g352600 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCTTTGGCGCGGGCGCTGTCGCAGCATCTGGCTTCTCATCCTCATCGT
Internal bar code:TGAACTTGCAATATCATAATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:560
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTGGTGCGGTCCCTACAT
Suggested primer 2:CGCCCAGAATAAATGTGCGG