| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.064221 |
| Chromosome: | chromosome 7 |
| Location: | 3228376 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334100 | GNOM | (1 of 1) K18443 - golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (GBF1); SEC7/BIG-type ARF-GEF | 3'UTR |
| Cre07.g334150 | (1 of 1) PTHR23097//PTHR23097:SF124 - TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATACCGTCACGAGTGCTAAGGCGTGGACGTTTCGTCTGCATGACACCG |
| Internal bar code: | CGGGAGGGTTCAAGCGGTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 948 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGAACGGCATACTGCAT |
| Suggested primer 2: | CATACATCCACGGTCGGAGG |