| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.064227 |
| Chromosome: | chromosome 12 |
| Location: | 2658724 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505500 | HTR17 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTTCTTTTTCTAGTCTCCCAATCAACTATCCAAGATGGCCCGCACCA |
| Internal bar code: | CGCGAGAACCTGTTTCCGTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3735 |
| LEAP-Seq percent confirming: | 86.5672 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTCTAGCCAGCTCTAGC |
| Suggested primer 2: | CGGATAGCGGGCTTGGTAAT |