| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064266 |
| Chromosome: | chromosome 7 |
| Location: | 659098 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317100 | POB18 | Proteome of basal body 18 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGGCGTGGAGAGGGGCCCGATGCCAGCAAAGCAGGCGGCGCCGAGTG |
| Internal bar code: | TTTTGCAATGTTTTTATCATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1460 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGAGTTTGAGACCGCCAC |
| Suggested primer 2: | CTGTTGACGTCCGTGTCTGA |