| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064268 |
| Chromosome: | chromosome 12 |
| Location: | 7257027 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558650 | EIF2B,EIF2BD | (1 of 1) K03680 - translation initiation factor eIF-2B subunit delta (EIF2B4); eIF-2B delta subunit | 5'UTR |
| Cre12.g558700 | FAP67 | Flagellar Associated Protein 67; (1 of 4) K00940 - nucleoside-diphosphate kinase (ndk, NME) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATAGTGCAAATTCATCCAATGATCATGCCTCTCCAACATAAACTGGAAT |
| Internal bar code: | GTTAACTACTGTATGTTTTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 447 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCACGACCATAGCATCCC |
| Suggested primer 2: | TATGCACGTGGCCACAGATT |