| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.064269 |
| Chromosome: | chromosome 3 |
| Location: | 1930682 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155150 | (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATGTCAATTAGCACAACCCCGCGGTCTCATATTTGGAGCAGCTGTGTT |
| Internal bar code: | TGATCACGGTATCTATGTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4262 |
| LEAP-Seq percent confirming: | 95.7447 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGCTAGATTTGCTGGTT |
| Suggested primer 2: | CTCAGGAGCCAGTGTCACAG |