| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064285 |
| Chromosome: | chromosome 2 |
| Location: | 1345678 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082865 | (1 of 10) PF07460 - NUMOD3 motif (2 copies) (NUMOD3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATTGTAATCAGTACCGAGTGGCCGCGCGTTGCTTTGGATAGCGGGAC |
| Internal bar code: | CTGCGGTTAAATTAATCCTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1599 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTTCGAAGCAGTGTGAG |
| Suggested primer 2: | CTTCTTGGCCGCCGATATCT |