| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064292 |
| Chromosome: | chromosome 9 |
| Location: | 882086 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g403450 | (1 of 6) PF12796//PF13920 - Ankyrin repeats (3 copies) (Ank_2) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGTAGATGTTCTTTATTTGGAGTTCTTTGCGCCGCGTCAAATGGGTC |
| Internal bar code: | GAATTAGACGTTGATAGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2797 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGTCAAGGGACATTCTCA |
| Suggested primer 2: | AAGCGGGAGTGCAGAATTGA |