| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.064325 |
| Chromosome: | chromosome 6 |
| Location: | 4743031 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279450 | MUT68 | (1 of 7) PTHR11584//PTHR11584:SF381 - SERINE/THREONINE PROTEIN KINASE // PROTEIN PQN-80, ISOFORM B; protein kinase | 5'UTR |
| Cre06.g800693 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCTGGGGTCCTTAGTTGGGGCTTGCAACTCGCCGTTGTGCTGCCAGTC |
| Internal bar code: | GAGTAAGGGTATCTTGTCTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2649 |
| LEAP-Seq percent confirming: | 97.8261 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTCGTCAAGCACATCGGT |
| Suggested primer 2: | GTGCGGCATGCTATTGAGTG |