| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.064342 |
| Chromosome: | chromosome 10 |
| Location: | 4689329 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452150 | (1 of 100) IPR001810 - F-box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCCCGGCCCGGTCCGGATCCACTTCAACCCGGCTGCCGCCGCCTGGT |
| Internal bar code: | TGAGGCCACTGGTACTCGACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2032 |
| LEAP-Seq percent confirming: | 0.740741 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 134 |
| LEAP-Seq n unique pos: | 135 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTAGCTCTTGTCCCCTCT |
| Suggested primer 2: | CCTCGATTGTCGTACGGAGG |