| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.064353 |
| Chromosome: | chromosome 3 |
| Location: | 6900447 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g198100 | BET1 | ER-to-Golgi Qc-SNARE protein, Bet1/mBET1 family (Qc.II); (1 of 1) K08504 - blocked early in transport 1 (BET1) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAACATAAATTACTACATAGCGTCATAAGCGAAATAAGCGTCATAAGCG |
| Internal bar code: | GGGCCGTTTGATCATCTGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5158 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAAGGTTCACCGGCAACC |
| Suggested primer 2: | TGCTAATGCCCCTGTTGGAG |