Insertion junction: CLIP2.064364_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.064364
Chromosome:chromosome 6
Location:3146749
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g275700 HFO4,HFO3 (1 of 33) K11254 - histone H4 (H4); Histone H4 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATCCAAAACTCGGTGTTTCTCAACACCACCCTGCCACATGGTTGCGAC
Internal bar code:CAGGAAACATCGTGTAGGAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2513
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GACACGATAAGCTAGCGCCT
Suggested primer 2:ATTTGTACCCAGTGGCCCAG