Insertion junction: CLIP2.064392_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.064392
Chromosome:chromosome 3
Location:3950101
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g171200 (1 of 3) 3.6.1.5 - Apyrase / ATP-diphosphohydrolase 3'UTR
Cre03.g171250 (1 of 1) IPR000104//IPR011989//IPR012677//IPR013083 - Antifreeze protein, type I // Armadillo-like helical // Nucleotide-binding alpha-beta plait domain // Zinc finger, RING/FYVE/PHD-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTGTGATCGCGTGTGAGCAGGCGCTGGACAGCGGTGTGTGCTGGTGCA
Internal bar code:GGTTTTTAGTGTCCGTTGGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:623
LEAP-Seq percent confirming:5.26316
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:54
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:CATGCGCCATCTGAAATCCG
Suggested primer 2:TAATCGAATTCTGGGGCGGG