Insertion junction: CLIP2.064392_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.064392
Chromosome:chromosome 3
Location:3950106
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g171200 (1 of 3) 3.6.1.5 - Apyrase / ATP-diphosphohydrolase 3'UTR
Cre03.g171250 (1 of 1) IPR000104//IPR011989//IPR012677//IPR013083 - Antifreeze protein, type I // Armadillo-like helical // Nucleotide-binding alpha-beta plait domain // Zinc finger, RING/FYVE/PHD-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCCATTACAAACGGGAACTTGCTTCTTATCACCCTCTGCTCTTGCCTG
Internal bar code:GTCGCTCAGACGGATCATAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:833
LEAP-Seq percent confirming:15.5556
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:TAATCGAATTCTGGGGCGGG
Suggested primer 2:CATGCGCCATCTGAAATCCG