Insertion junction: CLIP2.064412_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.064412
Chromosome:chromosome 17
Location:2766765
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g717900 PHC1 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAAGGTTCAACCATCCCGCCCGCGCAGCAGTGGGGGAAACACACAGGCAC
Internal bar code:TTTCAGCGGATAGCCCCCGGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2548
LEAP-Seq percent confirming:90.5063
LEAP-Seq n confirming:143
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:158

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGTGAAGCCACACACGTG
Suggested primer 2:AACTGCTGCCCTCTGTACAC