| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.064428 |
| Chromosome: | chromosome 6 |
| Location: | 738314 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g254917 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGTGTGGGCGTGTGGTTGGGGGGCGTGCATTGCACGTGCAAGGCGAG |
| Internal bar code: | GGTGGGTAGGAAGTGCGAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1233 |
| LEAP-Seq percent confirming: | 26.6667 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGTGTATGGTCCGGTG |
| Suggested primer 2: | GCACACCATCCAGTCACTCA |