Insertion junction: CLIP2.064460_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.064460
Chromosome:chromosome 3
Location:8641700
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g209953 (1 of 11) IPR001471//IPR016177//IPR031112 - AP2/ERF domain // DNA-binding domain // AP2-like ethylene-responsive transcription factor 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCATCGCCACGCCAGTGGCCTTACCAGTGCACCTGTCAGACGCTAGTG
Internal bar code:TCCATGCTGAGTTTACGATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1684
LEAP-Seq percent confirming:95.2381
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTTTGTCGTAGATGCGC
Suggested primer 2:CTTTGGTGCCCTCATGCCTA