| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064469 |
| Chromosome: | chromosome 17 |
| Location: | 2410508 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g714450 | KUP3 | (1 of 5) K03549 - KUP system potassium uptake protein (kup); Potassium ion uptake transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGCCACCCTGGCCTCCGTGGTAGCCAGCCAGGCGCTTATCACCGGCTG |
| Internal bar code: | ACTCGGACGTGGACCAATAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 707 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTGTGTGGGTTGTTTAC |
| Suggested primer 2: | CCAAGTGCGGCCATTAATCG |