| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064472 |
| Chromosome: | chromosome 1 |
| Location: | 4711290 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032600 | HPAT1,CGL25 | (1 of 14) PF14295 - PAN domain (PAN_4); Hydroxyproline O-arabinosyl transferase 1 | 3'UTR |
| Cre01.g032650 | TAL1 | Transaldolase; (1 of 1) PTHR10683//PTHR10683:SF6 - TRANSALDOLASE // TRANSALDOLASE-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCTATGTGTAACGGTGGCTTGTTTTTTTGTCCCCAAGTAAATTTTGAG |
| Internal bar code: | TTACCTATGGACCAGTAAGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3261 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAATCACGTGAGGAGGCC |
| Suggested primer 2: | CACAGGCAAGCCCTTCTACA |