Insertion junction: CLIP2.064477_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.064477
Chromosome:chromosome 17
Location:5201036
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g736950 (1 of 1) PF11265 - Mediator complex subunit 25 von Willebrand factor type A (Med25_VWA) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGGATTGCCAAGTTGCGCAGTGTCCAGACGCAACTAGCCAACTACATC
Internal bar code:CATCTGTGGGGGTAGGATTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:675
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTATGGGCGCTTGCTTGATG
Suggested primer 2:ACGCCATCCTATCATCAGCG