| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.064483 |
| Chromosome: | chromosome 3 |
| Location: | 5010260 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179750 | (1 of 14) PF00892 - EamA-like transporter family (EamA) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTAGGGAGACGCGTCCGAGTCAGAATCACGTAGCTTCCTCCATCGCC |
| Internal bar code: | AAGTCCTGCGACGTGGGCGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1617 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGTACGCGCACTTAGTT |
| Suggested primer 2: | GGCAGCAGGAACAGGTACTT |