| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.064491 |
| Chromosome: | chromosome 3 |
| Location: | 8243360 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199900 | EIF4E | Eukaryotic translation initiation factor 4E; (1 of 1) K03259 - translation initiation factor 4E (EIF4E) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCGCCCAGCATGGCACATGGGCACGTGACACTTCACCTGCTTCTATA |
| Internal bar code: | CTGGATAGAAATACGCTTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3018 |
| LEAP-Seq percent confirming: | 96.9231 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGACCGCTACATGGTGT |
| Suggested primer 2: | CCTCCTTCTCCACTGCCTTG |