Insertion junction: CLIP2.064492_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.064492
Chromosome:chromosome 6
Location:2465145
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g269000 (1 of 1) PF09493 - Tryptophan-rich protein (DUF2389) (DUF2389) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAAAAACAAAGCGCAACCACATGCGCTGTTTTTATAAAGTTGCTGGGCTC
Internal bar code:ACGGGATATAATGCGATTCCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1851
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GAAATTGAAGATCGGGGCGC
Suggested primer 2:ACTCGGCTGATTTGCAGTGA