Insertion junction: CLIP2.064503_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.064503
Chromosome:chromosome 6
Location:1646459
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g262000 SNR5 SNARE-associated Golgi protein; (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTAGTAGATAACCAACTCGCGTTTGCATGAAAGTCATCGAACCTCGAA
Internal bar code:GCCAAAAATGGGCGTGACGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1467
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTCTGGAAGGAAGGGCAG
Suggested primer 2:AATGTGTGAGTTCGCTCGGT