Insertion junction: CLIP2.064503_4

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.064503
Chromosome:chromosome 6
Location:1646476
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g262000 SNR5 SNARE-associated Golgi protein; (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACCACAATTCCTAATTGCTGACCAATAACCATTCGACGCGACAATTAC
Internal bar code:CTAGTCGTAGACTAAAACGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1067
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AATGTGTGAGTTCGCTCGGT
Suggested primer 2:CAGTCTGGAAGGAAGGGCAG