Insertion junction: CLIP2.064505_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.064505
Chromosome:chromosome 17
Location:1881486
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g802046 (1 of 7) PF00415//PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1) // Regulator of chromosome condensation (RCC1) repeat (RCC1_2) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAGCACCGGCACACATGCAATTCATTATCATTTGATATGCATTTACTC
Internal bar code:TCCCGCGGGTATTATGGGCCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2190
LEAP-Seq percent confirming:78.2609
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCATCTGAAACACGAGGA
Suggested primer 2:ACTGTGTCCCGCAATGATGT