Insertion junction: CLIP2.064513_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.064513
Chromosome:chromosome 8
Location:2114672
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g800917 3'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACAATCGTGCCACCACATACGTGCCACTACGGTTGTGCCGTACACACG
Internal bar code:CTTATCGTGACTGGCTTACTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:409
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGGGCCTAGTCAACCAGG
Suggested primer 2:ATCAACTCCTGCATAGCCGG