| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.064518 |
| Chromosome: | chromosome 12 |
| Location: | 6467638 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537581 | (1 of 1) 3.5.4.10 - IMP cyclohydrolase / Inosinicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGATGCACCCTCCTCCAGCCTCCTTCCCCTCCATACCGCCCCGTCCCTC |
| Internal bar code: | CCCGTTAAGAATGAGAATGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 88 |
| LEAP-Seq percent confirming: | 8.69565 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAAGGGCCTGAAGGGTAC |
| Suggested primer 2: | TCGGGCACATGACACATTGA |