| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.064522 |
| Chromosome: | chromosome 8 |
| Location: | 1543351 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365150 | FAP320,CSE7 | Coiled-Coil Flagellar Associated Protein 320 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCATGAGAGCATTGGTAAAAGCGGACAACACGAACCCAATCCAAGTGA |
| Internal bar code: | GGCGCGCACGAGTCCAAGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2749 |
| LEAP-Seq percent confirming: | 26.1905 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTGCCATTCACATCATG |
| Suggested primer 2: | TGCTGTCTGTGGAGCTTCTG |