Insertion junction: CLIP2.064540_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.064540
Chromosome:chromosome 11
Location:1291185
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467701 3'UTR
Cre11.g467702 MRPS3,uS3m (1 of 3) PF00189 - Ribosomal protein S3, C-terminal domain (Ribosomal_S3_C); Mitochondrial ribosomal protein S3 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTATTCCTATCGGTAGCGCGCCGACGCAGTGCCCCTGCCGCATCGTTGGC
Internal bar code:CCGCGGGTATACGAATGTCTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1368
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGGACGAGTGCGAATTGT
Suggested primer 2:TTATGCATGTGCTTACGCGC