| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.064541 |
| Chromosome: | chromosome 16 |
| Location: | 6064655 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677800 | (1 of 1) PTHR21530//PTHR21530:SF1 - PHEROMONE SHUTDOWN PROTEIN // TRAB FAMILY PROTEIN | 5'UTR | |
| Cre16.g677850 | (1 of 4) PF09366 - Protein of unknown function (DUF1997) (DUF1997) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGTACTAGGACTGAGACACTAGTTATTAAATAAGTTATACACAATGG |
| Internal bar code: | GTGTTGATTGCATCCCGCGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 6.25 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCGAAAATCGAAGTCCTC |
| Suggested primer 2: | TTTAGGCCAACCAGCAGGAG |