Insertion junction: CLIP2.064545_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.064545
Chromosome:chromosome 4
Location:522047
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g217930 (1 of 1) PF06911 - Senescence-associated protein (Senescence) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAATACTTCAGCGCGAGACTGCTGCTGCAGCTTATCATTGCAGTGTTAC
Internal bar code:TGCTGTAACTATAAATGCAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1877
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:ACACCGGATCGCAAATGACT
Suggested primer 2:ACAGTAGTAGTGGCCCGACT