| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064547 |
| Chromosome: | chromosome 8 |
| Location: | 3065392 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375450 | SDR13 | (1 of 1) 1.1.1.243 - Carveol dehydrogenase; Short-chain dehydrogenase/reductase | 5'UTR |
| Cre08.g375500 | ATF1 | Glucosamine-fructose-6-phosphate aminotransferase; (1 of 1) 2.6.1.16 - Glutamine--fructose-6-phosphate transaminase (isomerizing) / L-glutamine-D-fructose-6-phosphate amidotransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTGCTTGCGACGAGGGGTCCAACTCCCCCTGCCAGCACCATGAAGCA |
| Internal bar code: | CCCAGGACATATTCAAAGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGTTTAGGGTTAGGGCC |
| Suggested primer 2: | TGCCAGATGTTGACGAACGA |