| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064549 |
| Chromosome: | chromosome 10 |
| Location: | 1978572 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432150 | (1 of 18) K08824 - cyclin-dependent kinase-like [EC:2.7.11.22] (CDKL) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTCCGAACGAAGGAAGATACGAGCGCTGCACGAGACTCGTCAGCAGAG |
| Internal bar code: | ATAATACTGATGAGTATCATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2603 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCAGGCGGAAGGTTGAGA |
| Suggested primer 2: | ATAGGGTGGCAAGACAGCAC |