| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064550 |
| Chromosome: | chromosome 10 |
| Location: | 1011297 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424850 | SPL5,PRP31 | (1 of 1) K12844 - U4/U6 small nuclear ribonucleoprotein PRP31 (PRPF31); Pre-mRNA-splicing factor | 5'UTR |
| Cre10.g424900 | PHX13,PFH1 | Prolyl 4-hydroxylase 1; (1 of 2) PTHR10869//PTHR10869:SF66 - PROLYL 4-HYDROXYLASE ALPHA SUBUNIT // OXIDOREDUCTASE, 2OG-FE(II) OXYGENASE FAMILY PROTEIN-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAATGTAATGAAAATGGAACTGTCACGCGGCAAATAACAAAGGCTCTGT |
| Internal bar code: | GTAAACAGTTGTGCCGTGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1192 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACACGCACACCTGAACTG |
| Suggested primer 2: | GGTGTCCGTACCAACCACAT |