Insertion junction: CLIP2.064561_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.064561
Chromosome:chromosome 10
Location:4416898
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g450450 FAP18 Flagellar Associated Protein 18; (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAATTTAAATGGCATACGGTAAACATGATTGTTTCTTTCAGCGACACCC
Internal bar code:ATTTACTTTTCAGAATCGCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:5861
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:138
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:138

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGTACGACGCCATGATCA
Suggested primer 2:CAACAATCAACCCGCTGCAA