Insertion junction: CLIP2.064564_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.064564
Chromosome:chromosome 2
Location:2811837
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g094450 PHC38,PHC21 Pherophorin-chlamydomonas homolog 38; (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGACTGCTAAGGAGTCTGTTACTCGCGGGCTGCAGCGACTCAGGTGCG
Internal bar code:ATGAATAATTTTCTCGGACGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1515
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCAATAAGAACGCGGAAG
Suggested primer 2:GCTTGCGCGATCTTGCATTA