Insertion junction: CLIP2.064572_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.064572
Chromosome:chromosome 9
Location:2869860
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre09.g390700 POB1 Proteome of basal body 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTTTTAAAGCACACGAGTGACAAGCCTGAACAGAGGCCGACGCAACAC
Internal bar code:CGGAACGATAGCAGTTTCTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:137
LEAP-Seq percent confirming:27.0833
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:CGCAGTGGAAGGAGAAGAGG
Suggested primer 2:GGGACACTGCAGGTAACACA