| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.064574 |
| Chromosome: | chromosome 17 |
| Location: | 4372689 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730483 | (1 of 1) IPR000104//IPR016181 - Antifreeze protein, type I // Acyl-CoA N-acyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACGTTACTCGCGTGCAGGGGCCGCAACACTGCTTGCGCGCTCCACCAA |
| Internal bar code: | GCTTCTGGAGAACAAGTGACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1462 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGGGAGATGGGTCAGGT |
| Suggested primer 2: | GAACTGAGGGTGGCGTGTAA |