| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.064594 |
| Chromosome: | chromosome 16 |
| Location: | 6742193 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672800 | VTC1,GMP1 | GDP-mannose pyrophosphorylase; (1 of 1) 2.7.7.13//2.7.7.22 - Mannose-1-phosphate guanylyltransferase / GTP-mannose-1-phosphate guanylyltransferase // Mannose-1-phosphate guanylyltransferase (GDP) / GDP-mannose pyrophosphorylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGCCGGTTCAGCTTTGCCGATAGTGTTGGCGATTGTGCACAGAGGCTC |
| Internal bar code: | GGCACAAGGGATGAGGAAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3544 |
| LEAP-Seq percent confirming: | 66.0377 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTCTGCAGGATGAGCTG |
| Suggested primer 2: | GTGTGCAACTGTTGACGCAT |