| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064598 |
| Chromosome: | chromosome 2 |
| Location: | 8478860 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141950 | MOT19 | (1 of 5) PF03765 - CRAL/TRIO, N-terminal domain (CRAL_TRIO_N); Predicted protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGATGCAGAGTCCCTCTTGAGATGGTGTGGACTCAGGGGCGCATACAG |
| Internal bar code: | CCTGTGTAGCGCGAAACTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1159 |
| LEAP-Seq percent confirming: | 9.52381 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTCCGGGAACTGCTCTC |
| Suggested primer 2: | GGTTTCTGGTCGAGCATGGA |