Insertion junction: CLIP2.064598_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.064598
Chromosome:chromosome 2
Location:8478861
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g141950 MOT19 (1 of 5) PF03765 - CRAL/TRIO, N-terminal domain (CRAL_TRIO_N); Predicted protein 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCATCCTGTTGGCCCTGCGCTGAGTGAGTTTGTTGCTACACGCTACAC
Internal bar code:CCTGTGTAGCGCGAAACTCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:976
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTTCTGGTCGAGCATGGA
Suggested primer 2:CATCTCCGGGAACTGCTCTC