Insertion junction: CLIP2.064611_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.064611
Chromosome:chromosome 12
Location:4941497
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g524400 (1 of 1) IPR003128//IPR007122//IPR029919 - Villin headpiece // Villin/Gelsolin // Protein flightless-1 3'UTR
Cre12.g524450 PTP3 Protein tyrosine phosphatase; (1 of 1) PF00102//PF00782 - Protein-tyrosine phosphatase (Y_phosphatase) // Dual specificity phosphatase, catalytic domain (DSPc) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTACAATTGGTTCAATCCATCATGCGTAGCAAACAAGATTACGCAAG
Internal bar code:ACTAGGCTAGTGAATGGCAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3095
LEAP-Seq percent confirming:97.8723
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:GAATGGACCGCGATGCTTTC
Suggested primer 2:CTGGCGCAGATATGTTTGCC