| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064618 |
| Chromosome: | chromosome 16 |
| Location: | 3695854 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679876 | (1 of 1) PTHR15859//PTHR15859:SF4 - SETA BINDING PROTEIN 1 // SUBFAMILY NOT NAMED | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTGCGCGAGTTTTGCGTGAATTCCCCGCCAGTTTTTTCCGAGTTCCCG |
| Internal bar code: | GGCGGCTTAACGTAATAACGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2209 |
| LEAP-Seq percent confirming: | 17.9487 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGTGTGAGCAGGTAGTT |
| Suggested primer 2: | CTTCTACTCTGAGCGAGCGG |