| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064631 |
| Chromosome: | chromosome 13 |
| Location: | 369830 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801514 | SDH6,SDHAF1 | Succinate dehydrogenase assembly factor 1; (1 of 1) K18167 - succinate dehydrogenase assembly factor 1 (SDHAF1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGAACGTATTCGCGAAGGTCTGCGCGCTCACTTTCAGGCTTCGATCT |
| Internal bar code: | CGGCGTGGTGTTCAACATCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 888 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCACGCGTTCAAGCTCA |
| Suggested primer 2: | ATGGACACCCGCAGATTGAG |